目的：获得RECK基因GFP融合蛋白表达载体并使其在脑胶质瘤细胞SHG44中表达。
Objective: To obtain GFP-RECK expression vector and to express it in glioma cell line SHG44.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
结果从活化的人白细胞中克隆到IDO基因编码区全长，并构建了IDOEGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
结论：成功构建了人ip - 10融合蛋白表达载体，并纯化得到具有活性的IP - 10融合蛋白，为进一步研究IP - 10的功能提供了重要的实验材料。
CONCLUSION: We successfully construct IP-10 fusion protein expressing vector and get the fusion protein with high bioactivity, which provides essential materials for the future studies on IP-10.
结果：将编码人单核细胞趋化蛋白—1（MCP—1）基因克隆至融合表达载体pGEX—2T中，DNA测序证实正确。
Results: A gene fragement encoding MCP-1 was cloned into the fusional expression vector PGEX-2T. DNA sequencing indicated that it was correct.
将胰岛素原基因融合到金色葡萄球菌蛋白a的基因上，构建成大肠杆菌中基因融合的外分泌表达载体。
The secretion expression vector of fusion gene in E. coli has constructed by fussing the proinsulin gene to the gene of staphylococcal protein a.
结论：构建了8r - MUC1核心肽融合蛋白原核表达载体并成功表达与纯化出具有生物学活性的融合蛋白。
Conclusion the prokaryotic expression vector of 8r-muc1 core peptide fusion protein has been constructed, and the fusion protein with biological activity has been successfully expressed and purified.
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
目的：构建融合重组表达载体并对其进行诱导表达和蛋白纯化以获得大量重组融合蛋白。
AIM: to construct the recombinant expression vector and to obtain large amount of mouse Cyclin D1 protein fused to PTD.
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