目的:获得RECK基因GFP融合蛋白表达载体并使其在脑胶质瘤细胞SHG44中表达。
Objective: To obtain GFP-RECK expression vector and to express it in glioma cell line SHG44.
目的构建克隆载体,分析隐匿性乙型肝炎病毒S基因的突变情况,构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
结果从活化的人白细胞中克隆到IDO基因编码区全长,并构建了IDO EGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
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