研究人员通过建立动物肝脏再生模型和肝细胞原代培养模型，逐渐发现多种效应因子。
By study on animal models of liver regeneration and primary hepatocytes culture, investigators have proved many types of effective factors.
目的：探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
方法将营养琼脂、小牛血清、抗生素按一定比例制成固体支架，取乳兔肝细胞，作原代培养，观察细胞在固体支架上生长繁殖情况。
Methods Hepatocytes obtained from the new born rabbit were cultured on the AGAR scaffold made up of AGAR, embryo cattle strum and antibiotics, then observed the growth and proliferation of the cells.
目的探讨体外原代培养大鼠肝细胞的分离方法。
ObjectiveTo explore the isolating methods of rat hepatocytes for primary culture.
设计原代培养的乳猪肝细胞构建生物反应器，并观察肝细胞生长特点，进行体外灌流实验。
This article presents the design of a bioreactor using hollow fiber membrane and isolated hepatocytes of suckling pigs, and the experimental study of its efficacy in vitro.
目的通过比较EGF, EGF-雌激素以及雌激素对原代培养肝细胞DNA合成的影响，探讨用egf治疗肝细胞损伤的可能性。
Objective This test compares the effect of EGF and EGF-estrogen and estrogen on the primary culture hepatocytes's DNA synthesis to probe the possibilities of using EGF in treating hepatocytes' damage.
目的通过比较EGF, EGF-雌激素以及雌激素对原代培养肝细胞DNA合成的影响，探讨用egf治疗肝细胞损伤的可能性。
Objective This test compares the effect of EGF and EGF-estrogen and estrogen on the primary culture hepatocytes's DNA synthesis to probe the possibilities of using EGF in treating hepatocytes' damage.
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