• 肝细胞原代培养(primary culture of liver cell)操作步骤 • 淋巴细胞培养(Lymphocyte culture)条件选择和失败原因
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...肝细胞原代培养[gap=1809] hepatocyte growth factor; epidermal growth factor; c-met; carbon tetrachloride; primary hepatocyte culture...
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研究人员通过建立动物肝脏再生模型和肝细胞原代培养模型,逐渐发现多种效应因子。
By study on animal models of liver regeneration and primary hepatocytes culture, investigators have proved many types of effective factors.
目的:探索原代培养大鼠肝细胞的最佳分离方法。
Aim: To explore the best isolation methods of rat liver cells from primary culture.
方法将营养琼脂、小牛血清、抗生素按一定比例制成固体支架,取乳兔肝细胞,作原代培养,观察细胞在固体支架上生长繁殖情况。
Methods Hepatocytes obtained from the new born rabbit were cultured on the AGAR scaffold made up of AGAR, embryo cattle strum and antibiotics, then observed the growth and proliferation of the cells.
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