目的用磁激活细胞分选技术富集并定量分析结直肠癌患者外周血循环中的癌细胞。
ObjectiveTo enrich and quantitatively analyze peripheral circulative carcinoma cells by magnetic activated cell sorting (MACS) in colorectal cancer patients.
目的探讨利用荧光激活细胞分选技术获得有效重组逆转录病毒包装细胞系的方法。
Objective To develop a method for acquisition of efficient and stable retroviral packaging cells on the basis of fluorescence-activated cell sorting (FACS) technique.
然后通过免疫磁性细胞分选技术分离出播散的肿瘤细胞并进行培养，对培养成活的肿瘤细胞在鉴定来源的同时进行生物学活性检测，从细胞学角度判断入血肿瘤细胞形成转移灶的能力。
Secondly, we separated the tumor cells in blood samples by IMS and cultured it carefully, activities of the tumor cells has been tested to confirm their ability to form a successful metastasis.
结果①流式细胞术分选后共聚焦显微镜分析技术（PSC）能同时、准确地鉴别不同细胞周期时相的凋亡细胞和非凋亡细胞。
Results Post-sorting confocal (PSC) method could exactly distinguish apoptotic and non-apoptotic cells in the specific cell cycle phases at the same time.
结果电融合与磁性分选技术相结合可获得95%的融合细胞纯度。
Results The higher cell fusion efficiency can be obtained with electroporator and about 95% purity of fusion cells achieved by magnetic cells sorting unit.
结果电融合与磁性分选技术相结合可获得95%的融合细胞纯度。
Results The higher cell fusion efficiency can be obtained with electroporator and about 95% purity of fusion cells achieved by magnetic cells sorting unit.
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