...注射感染BALB/c小鼠,24h后取小鼠心脏血和肺组织,分离出细菌,利用荧光激活细胞分选技术(fluorescence-activated cell sorting,FACS)分选收集有荧光的细菌,最后共挑取64个菌落进行生物信息学分析。
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目前分离CTCs的标准方法是基于肿瘤细胞表面上皮性抗原的磁性激活细胞分选技术(magnetic-activated cell separation,MACS)。由于缺乏针对肝癌细胞表面特异性抗原的单克隆抗体,迄今鲜见MACS用于分离循环肝癌细胞的报道。
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...染BALB\c小鼠,24h后取小鼠心脏血和肺组织,分离出细菌,利用荧光激活细胞分选技术(fluorescence-activated cell sorting,FACS)分选收集有荧光的细菌,最后共挑取64个菌落进行生物信息学分析。
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流式细胞分选技术 fluorescence activated cell sorting ; FACS
荧光活化细胞分选技术 fluorescence activated cell sorting ; FACS
磁性细胞分选技术 Magnetic actived cell soring
荧光激活细胞分选技术 fluorescence-activated cell sorting
目的用磁激活细胞分选技术富集并定量分析结直肠癌患者外周血循环中的癌细胞。
ObjectiveTo enrich and quantitatively analyze peripheral circulative carcinoma cells by magnetic activated cell sorting (MACS) in colorectal cancer patients.
目的探讨利用荧光激活细胞分选技术获得有效重组逆转录病毒包装细胞系的方法。
Objective To develop a method for acquisition of efficient and stable retroviral packaging cells on the basis of fluorescence-activated cell sorting (FACS) technique.
然后通过免疫磁性细胞分选技术分离出播散的肿瘤细胞并进行培养,对培养成活的肿瘤细胞在鉴定来源的同时进行生物学活性检测,从细胞学角度判断入血肿瘤细胞形成转移灶的能力。
Secondly, we separated the tumor cells in blood samples by IMS and cultured it carefully, activities of the tumor cells has been tested to confirm their ability to form a successful metastasis.
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