• 高速离心、分段亲和、超阴离子交换层析法,胎盘中分离纯化出细胞生长因子

    A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.

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  • 结论肺癌相关抗原n 35一种重要肿瘤相关抗原,其功能很可能肿瘤细胞无限制增殖活动有关。麦芽凝集素亲和层析法是纯化肺癌相关蛋白n 35简便有效途径。

    ConclusionThe lung cancer associated antigen N35 might be an important tumor associated antigen and related with the proliferation of cancer cells as a role of tumor cell growth regulator.

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  • 采用亲和离子交换层析分离纯化细胞增殖实验测定表达蛋白生物活性。

    Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.

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  • 建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞RT-PCR克隆人抗体基因噬菌体呈现技术构建人源抗体策略

    Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.

    youdao

  • 建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞RT-PCR克隆人抗体基因噬菌体呈现技术构建人源抗体策略

    Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.

    youdao

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