用高速离心、分段盐析、亲和层析、超滤和阴离子交换层析法，从牛胎盘中分离纯化出一种肝细胞生长因子。
A kind of hepatocyte growth factor was purified from cow placenta through high speed centrifugation, salting out, affinity chromatography, ultrafiltration and anion exchange chromatography.
结论肺癌相关抗原n 35是一种重要的肿瘤相关抗原，其功能很可能与肿瘤细胞无限制增殖活动有关。麦芽凝集素亲和层析法是纯化肺癌相关蛋白n 35简便有效的途径。
ConclusionThe lung cancer associated antigen N35 might be an important tumor associated antigen and related with the proliferation of cancer cells as a role of tumor cell growth regulator.
采用亲和层析和离子交换层析分离纯化，以细胞增殖实验测定表达蛋白的生物活性。
Affinity chromatography and ion exchange chromatography were applied in isolation and purification, and the bioactivity of expression protein was determined in cell proliferation test.
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