应用单细胞凝胶电泳技术,研究了烷基酚类化合物对离体小鼠睾丸细胞DNA的损伤作用。
The single cell gel electrophoresis technique (SCGE) was used to detecte the DNA damage of spermary cell in mice, which were induced by 9 tested environmental endocrine disrupting chemicals.
采用单细胞凝胶电泳检测小鼠睾丸细胞DNA受损程度,显示DNA迁移细胞数和彗尾长度上升。
DMA damage were detected by single-cell gel electrophoresis assay. After exposure the relative tail length of the comets and cells with DNA migration were increased.
研究结果提示,精索静脉曲张睾丸功能障碍可能与血清睾酮浓度低下及睾丸细胞核雄激素受体反应异常有关。
The authors suggest that the disturbed function of testes in rats with varicocele may be related to lower values of serum testosterone and abnormal reaction of NAR in testes.
本研究旨在构建稳定表达T7RNA聚合酶的猪睾丸细胞系(ST细胞系),用于猪瘟病毒等的反向遗传操作。
This study was aimed to establish a ST-based cell line stably expressing the T7 RNA polymerase, which can be used in the reverse genetic manipulation of CSFV and other swine RNA viruses.
本研究用犊牛睾丸细胞及MDBK细胞从我国内蒙古自治区达拉特旗发病的进口小尾寒羊唇病理痂块中分离到一株病毒,并对其进行连续传代至病变稳定。
From the scab of the sick sheep , one strain of virus was isolated using Bovine Testicle cell and MDBK cell, then, it was not taken cell passaged until the CPE is order .
研究人员表示在过去的30到50年,男性睾丸生殖细胞癌发病率出现显著和持续增加,尤其是在斯堪的纳维亚国家。
During the last 30 to 50 years, the researchers said, there has been a notable and continued increase in the incidence of testicular germ cell cancers among men, especially in Scandinavian countries.
然而在这项新的研究中,研究人员能够从男性的睾丸组织提取细胞,分离干细胞,使之成为能够分泌胰岛素的胰岛细胞。
In the new study, however, researchers were able to take cells from men's testicular tissue, isolate stem cells, and turn them into insulin-secreting islet cells.
结论MMP - 2、MMP - 9、TIMP - 1在人睾丸精原细胞瘤的浸润转移过程中起重要作用,有可能成为估计预后的指标之一。
Conclusion MMP-2, MMP-9 and TIMP-1 play an important role in the infiltrative metastasis of seminoma and may act as one of the factors to evaluate prognosis.
ACN对大鼠睾丸中ABP和抑制素基因表达水平有影响,提示丙烯腈对睾丸中支持细胞可能有损伤作用。
The results indicate that ACN could induce changes in the levels of ABP and inhibin gene expression in testes of male SD rats, that may damage Sertoli cells in testes.
主要观察指标:各组大鼠血清睾酮含量、睾丸生精细胞增殖细胞核抗原的表达比较。
MAIN OUTCOME MEASURES the content of serum testosterone and the expression of proliferating cell nuclear antigen of testicular spermatogenic cell of rats in each group.
以18天鸡胚睾丸为实验材料,经胶原酶和胰蛋白酶两步酶消化法得到生精上皮细胞悬液。
Testes from 18-day-old embryonic chickens were used to isolate seminiferous epithelial cells by sequential two-step enzyme digestion with collagenase and trypsin.
自从牛睾丸中提取出一种水溶性物,并发现可以抑制肥大的垂体去势细胞而命名为抑制素以来,关于抑制素的研究已有70余年的历史。
Inhibin is a kind of material with water-solubility from bull testicular, of which the name arises from its effect in inhibiting castration cells in pituitary.
结论1%焦亚硫酸钠喂食后,引起小鼠睾丸组织细胞损伤,提示1%焦亚硫酸钠对小鼠有生殖毒性作用。
Conclusion After a feeding of 1% sodium metabisulfite solution, testis tissue showed hydropic degeneration, that is, toxic effect on mice reproduction can be caused by overdose sodium metabisulfite.
进行了小白鼠的急性毒性试验、蓄积毒性试验、小白鼠的骨髓细胞微核试验、睾丸染色体畸变试验及鼠伤寒沙门氏菌的致突变试验。
The Study included five experiments, i. e, acute toxicity test and accumulative test of rats, bone-marrow micronucleus test and testis chromosomal aberration test of mouse, and Ames test.
③在A组样本的睾丸组织中,HBVDNA分布弥散,可广泛发现于生精上皮基底室和近腔室的生精细胞上;
In testicle of group A, HBV DNA can be widely found during the outer compartment of seminiferous epithelium and the inner compartment of spermatogenic cells;
目的探讨不同强度运动训练对大鼠睾丸组织NOS和细胞凋亡表达的影响及其与睾酮水平相关性。
Objective to investigate the effect of different physical training on NOS and apoptosis expression in the testicle tissue of rats and the possible mechanism for the change of testosterone.
目的探讨丙烯腈(acn)对睾丸精原细胞dna的损伤作用及经肝微粒体酶活化前后该损伤程度的差异。
Objective to explore acrylonitrile (ACN) induced DNA damage on Newt spermatogonia, and to compare the difference in DNA damages before and after microsomal enzyme activation.
目的: 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞,优化培养条件,建立饲养细胞系,为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目的:以人胎儿睾丸组织为供体,免疫缺陷小鼠为受体,研究人类睾丸生精细胞异种移植后的继续发育情况。
Objective: To investigate the development of xenografted primitive human germ cells by using fetal testicular tissues as donor tissues and an immunodeficient mouse as the recipient.
目的:探讨CD117在睾丸生殖细胞肿瘤中的表达及其在鉴别睾丸精原细胞瘤和非精原细胞瘤中的价值和生物学意义。
Objective:To evaluate the expression of CD117 in human testicular germ cell tumors and its value in the differential diagnosis of seminoma and nonseminoma.
最近研究表明,NO及NOS在雄性生殖系统分布广泛,NOS主要分布于睾丸间质细胞、支持细胞,少量分布于生精细胞。
The latest study showed that NO and NOS are widely distributed in male reproductive system. NOS is mainly distributed in Leydig′s cells and sertoli′s cells and a minor portion in spermatogenous cells.
结论全反式维甲酸可以抑制睾丸卵黄囊瘤细胞的增殖,抑制肿瘤增殖机制可能与下调VEGF的表达有关。
ConclusionATRA could inhibit proliferation of yolk sac tumor cell line, and down regulation of VEGF could be the inhibitory mechanism of ATRA on yolk sac tumor cell line in vitro.
采用冷胰酶消化法分离培养大鼠睾丸支持细胞,采用单细胞凝胶电泳法检测亚硝酸钠对大鼠睾丸支持细胞dna的损伤作用。
Method: The effects of sodium fluoride on DNA damage and repair in isolated male mice testicular cells were observed with single cell gel electrophoresis test (Comet assay).
文中就PAE对睾丸间质细胞的毒性作用作一综述,为预防PAE的生殖毒性提供理论依据。
This article reviews the toxic effect of PAE on Leydig cells, and is meant to provide a theoretical basis for the prevention of the reproductive toxicity of PAE.
结果血清及睾丸局部T水平随攻虫剂量增加而明显降低,同时T受体表达代偿性增高(P<0.05),尤其在精母细胞;
Results Serum and local T decreased significantly as the injected dose increased, while its receptors increased(P<0.05), especially in spermatocytes;
前三个发育阶段睾丸精原细胞HSP70阳性率比较在统计学上有显著性差异(P<0.01),其阳性细胞率在新生猴期最低,童猴后期最高。
There was significant difference(P<0.01)in the ratio of HSP70-positive spermatogonia before puberty, which was lowest in neonatal and highest in late infantile.
前三个发育阶段睾丸精原细胞HSP70阳性率比较在统计学上有显著性差异(P<0.01),其阳性细胞率在新生猴期最低,童猴后期最高。
There was significant difference(P<0.01)in the ratio of HSP70-positive spermatogonia before puberty, which was lowest in neonatal and highest in late infantile.
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