按照前述操作(6.7)进行有限稀释法检测。
Limiting dilution assay was performed as described previously (6, 7).
用有限稀释法，得到来源于同一细胞的亚细胞系克隆；
Limiting dilution was used to obtain subcell line clone fr om the same cells.
方法采用有限稀释法，检测细胞单克隆形成的数量及大小；
MethodsA clone formation assay was carried out to determine the clonogenicity and regeneration ability of cells.
应用有限稀释法，由小鼠srs- 82腹水瘤细胞系分离得到SAC克隆株系列。
A clone system designated SAC was established from a SRS-82 mouse ascitic turnout cell line by using limited dilution method.
利用胶原酶灌注梯密度离心分离肝星状细胞，用有限稀释法建立大鼠肝星状细胞株ig12。
Hepatic stellate cell was isolated with collagenase perfusion and ladder density centrifuge. Hepatic stellate cell line IG12 was cloned with limited diluted method.
采用有限稀释法对阳生孔进行亚克隆，重复3次，得到两株单克隆细胞株：2E8、3C6。
Positive clone were subcloned to get monoclone cell lines by limit dilution, repeat 3 times, then we got two positive monoelone cell lines named:2E8,3C6.
采用有限稀释法对阳生孔进行亚克隆，重复3次，得到两株单克隆细胞株：2E8、3C6。
Positive clone were subcloned to get monoclone cell lines by limit dilution, repeat 3 times, then we got two positive monoelone cell lines named:2E8,3C6.
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