有限稀释法是一种常用的克隆方法,是指将需要再克隆的细胞株自培养孔内吸出并作细胞计数,计出1mL的细胞数。常用来筛选融合的动物细胞。
按照前述操作(6.7)进行有限稀释法检测。
Limiting dilution assay was performed as described previously (6, 7).
用有限稀释法,得到来源于同一细胞的亚细胞系克隆;
Limiting dilution was used to obtain subcell line clone fr om the same cells.
方法采用有限稀释法,检测细胞单克隆形成的数量及大小;
MethodsA clone formation assay was carried out to determine the clonogenicity and regeneration ability of cells.
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