本研究旨在构建稳定表达T7RNA聚合酶的猪睾丸细胞系(ST细胞系)，用于猪瘟病毒等的反向遗传操作。
This study was aimed to establish a ST-based cell line stably expressing the T7 RNA polymerase, which can be used in the reverse genetic manipulation of CSFV and other swine RNA viruses.
本研究为建立NDV的反向遗传操作系统奠定了基础，同时对长链RT-PCR法的条件优化和简化克隆的构建提供了参考。
This research will helpful for further study of NDV reverse-genetic system, and the optimization of long RT-PCR will helpful for simplify the construction of clones.
本研究为建立NDV的反向遗传操作系统奠定了基础，同时对长链RT-PCR法的条件优化和简化克隆的构建提供了参考。
This research will helpful for further study of NDV reverse-genetic system, and the optimization of long RT-PCR will helpful for simplify the construction of clones.
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