NSNSV的反向遗传操作(Reverse genetic)是通过病毒基因组cDNA操作获得病毒的过程,是90年代后期出现的新型病毒学技术,是病毒学基础和应用研究极为重要的技术手段和工具...
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本研究旨在构建稳定表达T7 RNA聚合酶的猪睾丸细胞系(ST细胞系),用于猪瘟病毒等的反向遗传操作。
This study was aimed to establish a ST-based cell line stably expressing the T7 RNA polymerase, which can be used in the reverse genetic manipulation of CSFV and other swine RNA viruses.
本研究为建立NDV的反向遗传操作系统奠定了基础,同时对长链RT-PCR法的条件优化和简化克隆的构建提供了参考。
This research will helpful for further study of NDV reverse-genetic system, and the optimization of long RT-PCR will helpful for simplify the construction of clones.
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