从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
细胞传代20次后，仍生长良好。
观察原代和传代细胞的生长情况。
The primary and passages of retinal progenitors were observed.
培养的细胞可以稳定生长传代。
使用标准培养液的传代细胞均为阴性。
The cells, which were cultured in standard culture medium, were negative.
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采用机械方法传代，常规方法冻存和复苏。
The cells were passaged by mechanical methods, frozen and thawed by general procedure.
诚实是最丰富的传代物。
传代培养细胞可在1 ~2周汇合成片。
几次传代培养后，大多数具有遗传稳定性。
Most of them had inheritable stability after several generation.
异种移植瘤及其传代肿瘤保持遗传稳定性。
Xenotransplanted tumors and tumors after serial passage maintained the genetic stability.
随着传代次数增加，染色体不稳定性更明显。
Chromosomal instability increased as the cell passage increased.
几年来，我一直撰写、讲授和宣传代码生成技术。
For several years, I have written, lectured, and evangelized about code-generation techniques.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
因此ANITA是一个源于不同传代线的复合群。
Therefore, ANITA is a complex group originated from different lineages.
可以通过上传代码进行部署，还提供供应硬件的功能。
You can deploy by simply uploading your code, and there is provisioning of hardware.
目的探讨血管内皮细胞在传代过程中的衰老过程及机制。
Objective To study the process of aging and its potential mechanisms during passage of vascular endothelial cells.
最后，气管抽吸的原代细胞和之后的传代细胞有同样模式。
Finally, primary cells from tracheal aspirates behaved in an identical manner as later passage cells.
自北美发现第一例病例以后，病例是不断传代的，而且越传代越多。
Since the first cases in North America found that after the cases are continuously passaged, and the more passaged more.
方法用狗肾传代(MDCK)细胞和鸡胚双腔法进行流感病毒分离。
Methods The influenza virus isolation was performed by MDCK cells and embryonated eggs.
最后讨论了遗传代数与M -遗传代数、L -遗传代数之间的关系。
Finally, the relations between hereditary algebra, M-hereditary algebra and L-hereditary algebra are discussed.
方法切取肾母细胞瘤患儿肿瘤组织植入裸小鼠右前肢皮下，连续传代。
Methods Fresh human Wilms' tumour specimens were sliced and inoculated hypodermically into the forelimbs of nude mice.
目的探索CD 40反义rna对肿瘤传代细胞K562细胞株的影响。
Objective To explore the effects of CD40 anti sense RNA on K562 cell line.
结论：利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
早期传代细胞染色体数出现非整倍体和非稳定性畸变，同时有两个标记染色体；
There were aneuploid and instable structure changes in chromosomes, and two marker chromosomes at the same time.
实验得出结论：不同的直投发酵剂在不同的传代次数对酸奶的品质有不同的影响。
The conclusions were achieved that the different culture time of the different direct VAT inoculation had different effect on quality of yoghurt.
经反复传代，初步建立了人胚胎角膜上皮细胞系，并对其生物学特性进行了研究。
After human embryo corneal epithelial cell line was established through many cell generations, its biological character was researched.
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