从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
细胞传代20次后，仍生长良好。
观察原代和传代细胞的生长情况。
The primary and passages of retinal progenitors were observed.
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采用机械方法传代，常规方法冻存和复苏。
The cells were passaged by mechanical methods, frozen and thawed by general procedure.
异种移植瘤及其传代肿瘤保持遗传稳定性。
Xenotransplanted tumors and tumors after serial passage maintained the genetic stability.
随着传代次数增加，染色体不稳定性更明显。
Chromosomal instability increased as the cell passage increased.
几年来，我一直撰写、讲授和宣传代码生成技术。
For several years, I have written, lectured, and evangelized about code-generation techniques.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
目的探讨血管内皮细胞在传代过程中的衰老过程及机制。
Objective To study the process of aging and its potential mechanisms during passage of vascular endothelial cells.
最后，气管抽吸的原代细胞和之后的传代细胞有同样模式。
Finally, primary cells from tracheal aspirates behaved in an identical manner as later passage cells.
较离心法简便的聚已二醇法可用于沙眼衣原体的培养及传代；
The PEG handling is better than the centrifugation at the separated and culture passage of Ct.
方法用狗肾传代(MDCK)细胞和鸡胚双腔法进行流感病毒分离。
Methods The influenza virus isolation was performed by MDCK cells and embryonated eggs.
本文探讨了连续传代培养中营养对昆虫病原线虫繁殖力及产量的影响。
The effects of nutrition on fecundity and yield of nematode in continued culture were evaluated.
最后讨论了遗传代数与M -遗传代数、L -遗传代数之间的关系。
Finally, the relations between hereditary algebra, M-hereditary algebra and L-hereditary algebra are discussed.
方法切取肾母细胞瘤患儿肿瘤组织植入裸小鼠右前肢皮下，连续传代。
Methods Fresh human Wilms' tumour specimens were sliced and inoculated hypodermically into the forelimbs of nude mice.
目的探索CD 40反义rna对肿瘤传代细胞K562细胞株的影响。
Objective To explore the effects of CD40 anti sense RNA on K562 cell line.
结论：利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
早期传代细胞染色体数出现非整倍体和非稳定性畸变，同时有两个标记染色体；
There were aneuploid and instable structure changes in chromosomes, and two marker chromosomes at the same time.
实验得出结论：不同的直投发酵剂在不同的传代次数对酸奶的品质有不同的影响。
The conclusions were achieved that the different culture time of the different direct VAT inoculation had different effect on quality of yoghurt.
经反复传代，初步建立了人胚胎角膜上皮细胞系，并对其生物学特性进行了研究。
After human embryo corneal epithelial cell line was established through many cell generations, its biological character was researched.
成纤维细胞胶原的合成与分泌、以及正常传代星状细胞细胞内胶原合成也有抑制趋势。
The collagen production and secretionof fibroblasts, the intracellular collagen production of stellate cells was also inhibited to some extent.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
结果用该法培养细胞传代生长快，细胞纯度达98%以上，足以满足各种细胞试验需求。
Results VSMC was grown well and fast, the purity is more than 98% which can meet various cell tests.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
方法：取新西兰白色家兔晶体上皮细胞进行原代培养，细胞融合后用胰蛋白酶进行消化传代。
Methods New Zealand white rabbit lens epithelial cells were primarily Cultured. After the confluence, the cells were trysinized and sub-cultured.
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