从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
几年来，我一直撰写、讲授和宣传代码生成技术。
For several years, I have written, lectured, and evangelized about code-generation techniques.
英国阿伯丁大学的研究者们将人体的DNA与鸟类和鼠类的遗传代码进行比较之后，提出了这一发现。
The researchers from Aberdeen University made their discovery after comparing the DNA of people with the genetic code of birds and mice.
“我们希望能获得红杉中最大且最佳的遗传代表，”天使长古树档案组织的共同创立者大卫·米拉奇说。
"We want to get the biggest, best genetic representations of the species," said David Milarch, a founder of the Archangel Ancient Tree Archive.
我们不宣传代言可能会在这个网站上提到或描述的任何特定的测试，产品，程序，意见，或其他信息。
We do not endorse any specific tests, products, procedures, opinions, or other information that may be mentioned or described in this Website.
细胞传代20次后，仍生长良好。
采用机械方法传代，常规方法冻存和复苏。
The cells were passaged by mechanical methods, frozen and thawed by general procedure.
异种移植瘤及其传代肿瘤保持遗传稳定性。
Xenotransplanted tumors and tumors after serial passage maintained the genetic stability.
随着传代次数增加，染色体不稳定性更明显。
Chromosomal instability increased as the cell passage increased.
目的探索人心房肌细胞的原代及传代培养方法。
Objective To explore a method for primary culture and subculture of human atrial myocardial cells.
方法将人肝门部胆管癌标本进行细胞培养，传代。
Methods The fresh human hilar cholangiocarcinoma specimen was cultured.
目的探讨血管内皮细胞在传代过程中的衰老过程及机制。
Objective To study the process of aging and its potential mechanisms during passage of vascular endothelial cells.
方法用狗肾传代(MDCK)细胞和鸡胚双腔法进行流感病毒分离。
Methods The influenza virus isolation was performed by MDCK cells and embryonated eggs.
本文探讨了连续传代培养中营养对昆虫病原线虫繁殖力及产量的影响。
The effects of nutrition on fecundity and yield of nematode in continued culture were evaluated.
最后讨论了遗传代数与M -遗传代数、L -遗传代数之间的关系。
Finally, the relations between hereditary algebra, M-hereditary algebra and L-hereditary algebra are discussed.
中华医学会儿科学分会内分泌遗传代谢学组青春发育调查研究协作组。
The Pubertal Study Group of the Subspecialty Group of Endocrinologic, Hereditary and Metabolic Diseases, Society of Pediatrics, Chinese Medical Association.
方法切取肾母细胞瘤患儿肿瘤组织植入裸小鼠右前肢皮下，连续传代。
Methods Fresh human Wilms' tumour specimens were sliced and inoculated hypodermically into the forelimbs of nude mice.
相反，EGF反应性神经干细胞快速增殖，易于传代，较少迁移和分化。
In contrast, EGF-responsive neural stem cells proliferated more rapidly, migrated and differentiated less, and were passaged more easily.
结论：利用多次差速传代可从混合培养的原代细胞中获得纯化的牙囊细胞。
Conclusion: Dental follicle cells can be purified by several differential passages from the mixed primarily cultured cells.
结果：原代培养的RPE细胞含大量色素呈多角形，传代的细胞透明呈梭形。
Results: Primary cultured RPE cells contained large amount of pigments in polygonal shape. Passages of cultured RPE cells presented transparent spindle shape.
背景：人胚胎干细胞传代培养的关键是抑制其自发分化、保证细胞的全能性。
BACKGROUND: Key point for subculture of human embryonic stem cells is to inhibit spontaneous differentiation and make sure totipotency of cells.
兔角膜上皮和内皮细胞组织块原代培养，消化接种于人胚肺饲细胞瓶传代培养。
After primary culture, rabbit corneal epithelium and endothelium were digested and inoculated in bottle having hFLP feeder cells for serial passage.
早期传代细胞染色体数出现非整倍体和非稳定性畸变，同时有两个标记染色体；
There were aneuploid and instable structure changes in chromosomes, and two marker chromosomes at the same time.
结果人羊膜上皮细胞可以在体外成功的培养传代，体外可连续传8 ~10代。
Results Human amniotic epithelial cells were successfully cultured and passaged in vitro, and could be passaged successively for 8-10 times.
经反复传代，初步建立了人胚胎角膜上皮细胞系，并对其生物学特性进行了研究。
After human embryo corneal epithelial cell line was established through many cell generations, its biological character was researched.
方法从人胚胎海马区分离神经干细胞，采用无血清培养基，进行体外扩增培养、传代。
Methods The serum free culturing technology was used to isolate, culture and pass neural stem cells from embryonic human hippocampus.
结果用该法培养细胞传代生长快，细胞纯度达98%以上，足以满足各种细胞试验需求。
Results VSMC was grown well and fast, the purity is more than 98% which can meet various cell tests.
方法用狗肾传代MDCK细胞培养法观察儿茶提取物抑制甲型流感病毒的致细胞病变作用；
Methods The inhibitory cytopathic effects (CPE) of catechu extract on influenza A virus were observed by MDCK cell culture techniques.
方法分别自3周龄大耳白兔关节软骨和半月板分离软骨细胞，行单层传代培养和离心管培养。
Methods Chondrocytes isolated from articular cartilage and meniscus of rabbits aged 3 weeks were respectively passaged in monolayer and cultured in centrifuge tube.
方法：取新西兰白色家兔晶体上皮细胞进行原代培养，细胞融合后用胰蛋白酶进行消化传代。
Methods New Zealand white rabbit lens epithelial cells were primarily Cultured. After the confluence, the cells were trysinized and sub-cultured.
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