研究目的：克隆表达人肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化。
Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.
结果：研究中克隆表达的重组蛋白序列与公布的SARS病毒S1蛋白的序列相同。
Results:Sequencing analysis confirmed that the recombinant protein has the same sequence of natural SARS virus S1 subunit.
本论文工作的重点是与人类疾病相关的蛋白质的结构域的克隆表达及结构、功能的研究。
This thesis focus on the cloning, expression, purification and structural and functional studies of domains of human disease related proteins.
目的：对牛牙骨质附着蛋白进行N末端氨基酸序列分析，为牙骨质附着蛋白的克隆表达提供一定的理论依据。
Objective:To analyze the N-terminal amino acid sequence of bovine cementum attachment protein(CAP), which lay a theoretical foundation for cloning and expression of the CAP.
目的克隆表达人MAGE - 3基因片段(210 ~ 623位碱基)，以便研究其对MAGE - 3阳性恶性肿瘤的生物学作用。
Objective: To clone and express the MAGE-3 gene fragment (210 ~ 623nt) for researching its biological effects on MAGE-3 positive malignant tumors.
克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
乙酰-D-葡萄糖胺2-差向异构酶基因的克隆及表达。
Cloning and expression of N-acetyl-D-glucosamine2-epimerase.
但是可别指望它拥有一样的个性：它们只不过看上去想像，不过基因表达可能使克隆体令你大失所望。
But don't expect it to have an identical personality: it may look the same but genetic expression may turn it totally mental.
本论文应用基因工程的手段克隆、表达并纯化了人的肝再生增强因子基因。
This dissertation USES the gene engineering method to clone, express and purifies the human ALR gene.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
猴hcbp6同源基因的克隆化为研究不同种属来源的HCBP6基因的结构域功能、表达与调控等研究奠定了基础。
The successful cloning of monkey HCBP6 gene paves a way for the study of the relationship of structure and function of HCBP6 genes from different species.
目的：克隆造血干细胞因子并在CHO细胞中表达。
Objective: To study the expression of murine stem cell factor in CHO cells.
表观遗传修饰在基因表达和克隆胚的早期发育方面有重要作用。
Epigenetic modifications plays an important role in gene expression and the early development of cloned embryo.
建立了筛选质粒表达文库和克隆免疫原基因的技术方法。
We established the techniques of screening the plasmid expression library and cloning the immunogen genes.
木霉纤维素酶基因的克隆与表达研究进展。
The clone and expression of cellulase gene of trichoderma spp.
在现代分子生物学技术研究中，常常需要对已知位点的侧翼序列进行分析或克隆，以研究基因的遗传表达调控。
In modern research of molecular biology, we usually need to analyse or clone these flanking sequence in given sites, so as to study gene expression and control.
目的克隆与原核表达“牵引丝蛋白基因”单体六聚物，为开展具有不同长度系列的“牵引丝蛋白基因”单体多聚物的功能研究奠定基础。
Objective To clone and express the hexamer of the gene of spider dragline silk protein, as a foundation of further research on the functions of the dragline silk protein gene of different lengths.
结论：可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
目的：克隆并表达艰难梭菌毒素A羧基末端受体结合区基因。
AIM: to obtain the high expression of the gene coding for clostridium difficile toxin a receptor binding zone.
目的：克隆与表达辣根过氧化物酶同功酶c3 (HRPC3)基因。
Objective: To clone and express the horseradish peroxidase isozyme C3 (HRPC3) gene.
为研究该酶，克隆和表达该基因片段。
To study N-acetylglucosaminidase, clone and express the gene.
基因的表达检测证明，克隆菌株具有纤维二糖水解酶活力。
The gene expression test showed that the cloned strains expressed the enzyme activity of cellobiohydrolase.
对花粉发育及其特异基因的克隆、表达和调控进行了综述。
The cloning, expression and controlling of specific genes in developing pollen were reviewed briefly.
互补；克隆分子；序列分析；基因表达。
DNA, complementary; cloning, molecular; sequence analysis; gene expression.
目的：克隆和表达人源化小鼠抗人交联纤维蛋白单链抗体。
Objective: To clone and express humanized mouse ScFv against human cross linked fibrin (HScFv).
目的克隆srg基因、原核表达并鉴定。
目的：克隆及表达人胶质瘤特异性抗原MAGEE1基因片段。
AIM: To clone and express the testicular carcinoma antigen MAGE E1 gene in e.
获得了一个YAC OAT1编码的表达顺序克隆。
One expressed single copy sequence clone of YAC OAT1 was obtained.
结果显示：经g 418筛选后的阳性细胞克隆，为高表达VEGF的鼠成纤维细胞模型。
The results showed that the positive clones screened by G418 were mouse fibroblast cells with high expression level of VEGF.
结果：将编码人单核细胞趋化蛋白—1（MCP—1）基因克隆至融合表达载体pGEX—2T中，DNA测序证实正确。
Results: A gene fragement encoding MCP-1 was cloned into the fusional expression vector PGEX-2T. DNA sequencing indicated that it was correct.
应用推荐