半乳糖苷酶; 克隆表达; His?Tag; 复性 [gap=774]Key words】 Lactobacillus acidophilus; β?galactosidase; expression; His?
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一种不依赖钙离子的酸性α淀粉酶基因的克隆表达- 生物研究-生物谷 关键词: 酸性淀粉酶, 不依赖钙离子, 克隆表达, pKa [gap=1087]Keywords: Acid amylase, Ca-independent, Cloning and expression, pKa
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表达克隆 expression cloning ; cloning by expression ; Expression clone ; MTASER
基因克隆表达 genetic cloning and expression
克隆与表达 Clone and expression ; The Cloning And Expression
表达克隆化 expression cloning phenotype cloning
克隆和表达 clone and expression ; Cloning and expressing
基因克隆和表达 Gene clone and expression
基因克隆与表达 gene cloning and expression
克隆基因的表达 Expression of Cloned Gene
An express and clone vector pEB was constructed and expressed toxin01, toxin02, toxin03 and toxin04 successfully.
构建了一个高效克隆表达载体pEB,提高了基因表达载体构建效率。 通过这个载体成功表达了toxin01,toxin02,toxin03,toxin04四个基因,但这4个表达产物对双翅目害虫库蚊没有杀虫活性。
参考来源 - 苏云金芽胞杆菌Y41新型cry基因克隆及其质粒的分析ObjectiveDetecting, Cloning and Expression of KPC enzyme gene of Klebsiella pneumoniae.
目的:肺炎克雷伯菌中KPC酶基因的检测及克隆表达。
参考来源 - 临床分离肺炎克雷伯菌的耐药性及KPC型碳青霉烯酶的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
研究目的:克隆表达人肠激酶轻链编码基因,以期应用于融合蛋白的切割与纯化。
Objective: To clone and express the gene of human enterokinase light chain which would be used in the cleavage and purification of fusion proteins.
结果:研究中克隆表达的重组蛋白序列与公布的SARS病毒S1蛋白的序列相同。
Results:Sequencing analysis confirmed that the recombinant protein has the same sequence of natural SARS virus S1 subunit.
本论文工作的重点是与人类疾病相关的蛋白质的结构域的克隆表达及结构、功能的研究。
This thesis focus on the cloning, expression, purification and structural and functional studies of domains of human disease related proteins.
Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.
将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达
We're going to have DNA that we would like to make lots of copies of, or we're going to have DNA that we would like to get expressed in a cell, and we're going to put it in this region that's called the polylinker.
我们将那些需要得到很多拷贝数的DNA,或者需要在细胞中表达的DNA,整合到这个叫做多克隆位点的区域中
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