目的观察标记引物法、随机渗入标记法、末端转移标记法三种荧光标记法对寡核苷酸芯片杂交信号的影响。
Objective to observe the impact of three fluorescence marker methods of primer marker, random inleakage marker and terminal transfer marker to Oligonucleotide microarray hybridize signal.
方法：荧光标记的多态性微卫星引物d10s1265与83例结直肠癌的肿瘤和正常组织进行PCR反应。
Methods: D10S1265, a fluorescent labeled polymorphic microsatellite marker, was analyzed in 83 cases of sporadic CRC and normal tissue DNA by PCR.
结论：采用荧光标记引物进行复合扩增，结合PE310遗传分析仪自动分析，可以有效地区分各位点的扩增产物。
Conclusion: By co amplification using fluorescently labeled STR primers and detecting using PE310 genetic analyzer, the PCR products of these nine loci can be significantly distinguished.
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