目的观察标记引物法、随机渗入标记法、末端转移标记法三种荧光标记法对寡核苷酸芯片杂交信号的影响。
Objective to observe the impact of three fluorescence marker methods of primer marker, random inleakage marker and terminal transfer marker to Oligonucleotide microarray hybridize signal.
方法：荧光标记的多态性微卫星引物d10s1265与83例结直肠癌的肿瘤和正常组织进行PCR反应。
Methods: D10S1265, a fluorescent labeled polymorphic microsatellite marker, was analyzed in 83 cases of sporadic CRC and normal tissue DNA by PCR.
结论：采用荧光标记引物进行复合扩增，结合PE310遗传分析仪自动分析，可以有效地区分各位点的扩增产物。
Conclusion: By co amplification using fluorescently labeled STR primers and detecting using PE310 genetic analyzer, the PCR products of these nine loci can be significantly distinguished.
方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
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