方法将特异性引物从S85 46病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法：采用PCR技术，体外扩增STAT6分子的不同功能域，扩增后的目的基因分别构建于表达载体。
Methods Different domains of STAT6 were amplified by PCR technique and ligated respectively into the prokaryotic expression vector.
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