方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法：采用PCR技术，体外扩增STAT6分子的不同功能域，扩增后的目的基因分别构建于表达载体。
Methods Different domains of STAT6 were amplified by PCR technique and ligated respectively into the prokaryotic expression vector.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
目的探讨常见载体上的微量血痕dna的提取方法、PCR循环次数对STR扩增成功率的影响。
Objective To study the influence of different DNA extraction methods combined different PCR cycle Numbers on STR analysis from trace bloodstain on various carrier often seen in forensic area.
目的扩增木瓜凝乳酶基因，构建重组表达载体。
PurposeThe chymopapain gene was amplified, and the recombinant vector was constructed.
本文对食源性乳酸菌抗药性产生的原因，传递外获抗药性因子的载体及传递方式进行了综述，以期加强人们对抗药性菌株扩增的防范意识。
The paper stated formative reason, transfer vehicle and transfer ways of acquired antibiotic resistance in LAB. seas to intensify peoples' consciousness of avoiding antibiotic resistant bacteria.
本文对食源性乳酸菌抗药性产生的原因，传递外获抗药性因子的载体及传递方式进行了综述，以期加强人们对抗药性菌株扩增的防范意识。
The paper stated formative reason, transfer vehicle and transfer ways of acquired antibiotic resistance in LAB. seas to intensify peoples' consciousness of avoiding antibiotic resistant bacteria.
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