9.5.2 化学诱变(chemical mutagenesis) 诱发突变(induced mutation): 人为用物理 射线、化学诱变剂处理所诱发产生的突变。 化学诱变: 通过化学诱变剂引起的基因突变。
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关键词 : 水稻条斑病细菌 ; 化学诱变 ; dsp 基因 ; 功能互补 [gap=1322]Key words : Xanthomonas oryzae pv1 oryzicola ; mutagenesis ; dsp genes ; function complementation
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... 黄河上游:Upper Yellow River 化学诱变:chemical mutation 叶绿素a浓度:chlorophyll-a concentration ...
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Three varieties of upland cotton(Gossypium hirsutum L. ),which are G-20,N-156 and NK-20,were induced by three methods including EMS chemical mutation,14N heavy ions implantation and 60Coγ-rays radiation to select botany character,yield trait and fiber quality trait mutants.
采用EMS化学诱变、14N重离子注入及60Coγ射线辐照3种诱变方式处理冀棉20、农大156和农抗2号棉花品种,利用四分位法和系谱选择法筛选到植物学性状、产量性状和纤维品质性状突变体并采用SSR标记对突变体进行分子水平鉴定。
参考来源 - 棉花有益突变体的创制及突变性状的分子遗传学鉴定The traditional method of chemical mutagenesis can produce high-density induced mutations with similar genetic background.
传统的化学诱变方法,能诱发产生高密度的点突变,获得遗传背景相似的突变体。
参考来源 - 小麦大粒突变体的鉴定和相关cDNA片段的克隆Variations in number of chromosomes in callus by mutation treatment were observed. It was found that the ratio of diploid 、aneuploid and polyploid of induced callus were higher than the control.
9.初步建立了白桦愈伤组织化学诱变技术:在高浓度EMS短时间处理和低浓度EMS长时间处理条件下可获得到叶柄、叶片愈伤组织的半致死剂量:通过观察半致死剂量下愈伤组织细胞的染色体数目发现,诱变后细胞中单倍体、非整倍体及多倍体细胞比例均显著高于对照,总变异率由诱变前60%提高到89.93%,这说明EMS对白桦愈伤组织的诱变作用是显著的。
参考来源 - 白桦愈伤组织再生系统的体细胞无性系变异研究Error prone PCR and chemical mutation are used to generate gene diversity and a constitutive expression vector pBS-ks(sv)is used to creat the mutants library.
本文采用易错PCR和化学诱变来建立分子的多样性,以组成型表达载体pBS-ks(sv)作为载体建立突变体文库。
参考来源 - 新型βNew triploid line 99-3 of seedless Ningxia wolfberry is bred successfully by Ningxia Forestry Institute using advanced breeding approachese including a combination of cell and tissue culture technique with chemical induction of mutation as well as culture of young embryo.
采用组织培养技术与化学诱变相结合及幼胚培养等育种手段,初步选育出优良三倍体株系99-3。
参考来源 - 无籽枸杞新品种选育研究 New variety breeding of seedless Ningxia wolfberry·2,447,543篇论文数据,部分数据来源于NoteExpress
化学诱变育成农作物品种的新方法。
Chemical mutagenesis breeding is an new method to breed crop varieties by chemical mutagenesis.
他们中大多都利用基因工程或者其它的生物技术,例如化学诱变,来改善藻类的功能。
And many of them are using genetic engineering or other biological techniques, like chemically induced mutations, to improve how algae functions.
传统的化学诱变方法,能诱发产生高密度的点突变,获得遗传背景相似的突变体。
The traditional method of chemical mutagenesis can produce high-density induced mutations with similar genetic background.
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