...培养基：葡萄糖2％，蛋白胨2％，酵母提取物1％，pH值自然；固体培养基：液体培养基中添加2％琼脂，pH值自然；再生培养基（YEPDS）：固体培养基中添加0.7mol／l_， KC 1。体细胞中作用位置可能存在很大差别【l3J。
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The results indicated that response surface method was an effective way to optimize regeneration medium.
结果表明，响应面法是优化原生质体再生培养基的一种有效方法。
参考来源 - 响应面优化褐黄孢链霉菌原生质体再生培养基·2,447,543篇论文数据，部分数据来源于NoteExpress
采用麸皮再生培养基替代R2YE再生培养基，原生质体再生率、生长及筛选效果得到明显改善。
The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium.
低盐浓度的WPM培养基尽管对不定芽诱导没有优势，但可维持再生不定芽的正常生长。
Low-salt medium (WPM), while had no advantages over other media on the induction of adventitious buds, supported the continuous growth of adventitious buds of Mandshurican ash.
由此可见，不同激素浓度配比的培养基再生体系的建立影响不同。
So culture medium having different concentration ratio have different effect on regeneration system establishment.
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