采用麸皮再生培养基替代R2YE再生培养基,原生质体再生率、生长及筛选效果得到明显改善。
The effect of protoplast regeneration and screen was enhanced when R2YE medium was replaced by bran medium.
低盐浓度的WPM培养基尽管对不定芽诱导没有优势,但可维持再生不定芽的正常生长。
Low-salt medium (WPM), while had no advantages over other media on the induction of adventitious buds, supported the continuous growth of adventitious buds of Mandshurican ash.
利用水稻成熟胚为外植体,接种在NB培养基上,分别附加不同的外源激素,以诱导愈伤组织并促其分化,最终获得水稻再生植株。
The mature embryos were cultivated on NB media with different hormones, by using the mature embryos in rice as explants, and regeneration plants were obtained successfully.
结果表明,纤维二糖培养基及双层培养法比较适合灵芝原生质体再生。
The results showed that the cellobiose medium and double layer culture method were most suitable for protoplast regeneration of Ganoderma lucidum.
由此可见,不同激素浓度配比的培养基再生体系的建立影响不同。
So culture medium having different concentration ratio have different effect on regeneration system establishment.
其细胞壁再生不需特殊培养基。
The regeneration of cell walls did not need specific culture medium.
目的研究神经干细胞条件化培养基对脊髓损伤大鼠(SCI)皮质脊髓束(CST)再生的促进作用。
Objective Study the action of Neural stem cell (NSC) conditioned culture medium on promoting corticospinal tract (CST) in rats with injured spinal cord (SCI).
不同激素配比的分化培养基在诱使再生植株染色体倍性变异方面存在着明显差异。
The differentiation media with different rates of growth regulators demonstrated distinct difference in inducing ploidy variation for regenerated plants.
在茎尖再生体系建立的过程中,不同培养基上外植体丛生芽发生的方式和芽尖倍增数均不同。
In the process of established regenerate system from shoot meristems, the mode of tussockybud occurring and multiplication of shoot meristems were all different.
据此认为,提高培养基中铜元素浓度是一种增强水稻愈伤组织植株再生的简单和有效的方法。
Results of the present experiments indicate that raising copper concentration of the medium is a simple and effective method for the enhancement of plant regeneration in rice callus culture.
本论文的研究内容及结果如下:1。通过对分化和生根培养基的筛选,我们建立了高效的番茄再生体系。
The results are as follows:1. We first established an efficient tomato regeneration system by screening differentiation and rooting medium.
对油葵子叶外植体在含有不同激素浓度的培养基上进行了诱导、分化及成苗的研究,建立起一套完整的再生体系。
The factors that influence callus induction, shoot differentiation and growth of Helianthus annuus cotyledon were studied and an efficient regeneration system was established .
结果显示:影响银白杨植株再生的最主要因素是激素浓度和类型,其次是培养基种类和外植体类型;
The results show that the main factors affecting the efficiencies of regeneration system are hormone concentration and types as well as culture medium kinds and explants types.
用较高渗透压的培养基培养原生质体再生的细胞团及愈伤组织,可提高植株再生频率。
Using media with relatively high osmotic pressure in cul-turing protoplast-derived calli increased their plant regeneration frequency on subsequent differentiation media.
转移3 - 6周后,将愈伤组织进一步转移到添加吲哚乙酸(IAA)和6 -苄基嘌呤(bap)的MS培养基上,一些愈伤组织再生出植株。
When the obtained calli were further transferred onto MS medium supplemented with 3-indoleacetic acid (IAA) and 6-benzylaminopurine (BAP), some of them regenerated plants.
分化培养基上不同激素配比也影响其绿苗再生率。
The green shoot-bud regeneration rates of calli were affected by combinations of different auxins in regeneration medium.
为了建立高效稳定的甜瓜作物的植株再生体系,研究了甜瓜品种GT - 1在16种培养基上的植株再生情况。
In order to establish a stable and efficient plant regeneration system for Cucumis melon, plant regeneration of C. melon CV. GT-1 was studied on 16 culture mediums.
水稻组织再生植株的诱导能力因种类、器官、培养基等而异。
The inducibility of tissues of rice to regenerate plant lets varied according to the species, organs or media.
全量培养基比半量培养基更适合愈伤组织继代;MS基本培养基有利于种胚植株再生。
Full quality of basic media were more suitable to callus subculture than half quality of that. But MS basic medium was advantageous to plant regeneration of mature embryo.
胚性愈伤诱导率和植株再生率与培养基的种类、培养基中2,4 - D的浓度、小麦基因型及诱导和分化培养基中激素种类和浓度等有关。
Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of 2, 4-d.
MS培养基有利于再生植株的生根;
研究了前培养基中生长素、细胞分裂素等附加成分对愈伤组织的生长、根苗分化的影响和愈伤组织再生植株形成的途径。
The effects of pre-medium auxin, cytokinin etc. on the callus growth, root and shoot differentiations were investigated. The way of regeneration from callus was studied.
以美国黑树莓叶片作为外植体,以MS为基本培养基,添加不同浓度的6-BA、NAA或IAA进行愈伤组织诱导及植株再生试验。
The leaves of the blackberry as the explant, and MS contained different concentration of 6-BA, NAA or IAA as the media, the callus induction and the plant regeneration of the blackberry were studied.
再生植株在无激素的培养基上生长良好。
The regenerated plants grew well on MS medium without any hormone.
结果表明,2,4 - D是诱导体细胞胚胎发生的关键因素,当培养基中只含2,4 - D而不含或少含细胞分裂素(6 -BA)时,外植体经由体细胞胚胎发生途径形成再生植株。
The results showed the explants could regenerate via somatic embryogenesis when the medium contained only 2, 4-d without or with little content of 6-ba.
不同品种的植株再生在不同浓度有机添加物的分化培养基上表现不同;
Regeneration media added different concentration of organic nutrients influenced plant regeneration of different barley genotypes.
通过三因素三水平正交试验,对基本培养基、ZT及IAA对叶片不定芽再生的影响进行了研究。
Effects of basal media, ZT and IAA on adventitious shoot regeneration were studied through orthogonal design trial L9 (34).
通过三因素三水平正交试验,对基本培养基、ZT及IAA对叶片不定芽再生的影响进行了研究。
Effects of basal media, ZT and IAA on adventitious shoot regeneration were studied through orthogonal design trial L9 (34).
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