克隆位点指限制性内切酶的酶切位点
探针标记方法_生物站 。这些质粒中应该包括SP6、T3、或T7 RNA聚合酶的 RNA启动位点和,而这些启动位点则与多 克隆位点(multiple cloning sites,MCS)相邻。 4、RNA的寡脱氧核苷酸法。克隆质粒pSP64、pGEM-3、pGEM-4等含邻近MCS的
基于2个网页-相关网页
探针标记方法_生物站 。这些质粒中应该包括SP6、T3、或T7 RNA聚合酶的 RNA启动位点和,而这些启动位点则与多 克隆位点(multiple cloning sites,MCS)相邻。 4、RNA的寡脱氧核苷酸法。克隆质粒pSP64、pGEM-3、pGEM-4等含邻近MCS的
基于2个网页-相关网页
重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物,用自动测序仪测序鉴定克隆的正确性。
The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.
采用大引物方法,利用质粒多克隆位点两侧的普通测序引物作为旁侧引物,在单个PCR管内,经2个步骤共34个循环进行定点突变。
Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.
The rest of this, this sort of beige part of the molecule here is called the polylinker part.
剩下这部分,这部分淡棕色的分子叫做多克隆位点
How we do that will be clear in a few minutes, but this polylinker as is described down here is a site where you can clone in genes.
马上你们就清楚这该怎么做了,这里所说的多克隆位点,就是你能把基因片段插进来的位点
We're going to have DNA that we would like to make lots of copies of, or we're going to have DNA that we would like to get expressed in a cell, and we're going to put it in this region that's called the polylinker.
我们将那些需要得到很多拷贝数的DNA,或者需要在细胞中表达的DNA,整合到这个叫做多克隆位点的区域中
应用推荐