...Serum-free culture medium; Floating-culture system [gap=498]关键词: Lovo大肠癌细胞系; 细胞亚群; 肿瘤干细胞; 悬浮培养; 无血清培养基 ...
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Cells were divided into control group(cells in serum-free culture medium) , H_2O_2-treated group(cells in serum-free culture medium with 10、100、500 and 1000μM H_2O_2) and p38 MAPK inhibition group.
实验分为对照组(加无血清培养基)、H_2O_2处理组(无血清培养基中含10、100、500和1000μM H_2O_2)和p38MAPK干预组(加p38MAPK抑制剂SB203580)。
参考来源 - 氧化应激状态下肺泡Ⅱ型上皮细胞修复存活、凋亡的变化及p38MAPK信号转导通路的分子机制·2,447,543篇论文数据，部分数据来源于NoteExpress
Methods kidney cortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶- EDTA消化传代。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
受精卵在含血清 FBS 或 OCS 培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cell culture of serum free medium.
方法：采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元，观察氦氖激光照射对神经元生长发育的影响。
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