Methods kidney cortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶-EDTA消化传代。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
受精卵在含血清FBS或OCS培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cell culture of serum free medium.
方法：采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元，观察氦氖激光照射对神经元生长发育的影响。
Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.
结论目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。
Methods: We selected the best medium fitted for es cells culture, and analyzed the neural differentiation of es cells by comparing increased number of viable cells among different serum-free medium.
方法：比较ES细胞在不同培养基中的生长情况，分析ES细胞在不同时间分化形成神经细胞的比例。
Conclusions With the application of the "m" serum - free medium, the technique of Schwann cell culture was simplified and the contamination of fibroblasts was effectively controlled.
结论应用“M”无血清培养液简化了雪旺细胞的培养技术，有效地控制了成纤维细胞的污染。
Instect cell culture media were optimized carefully to find new medium for special cell strain and serum free media was manufactured commercially for suspension cell mass production;
通用的昆虫细胞培养基配方的不断优化，特定昆虫细胞株培养基的研制和适用于大规模悬浮培养的无血清培养基的商品化；
The present invention provides a serum free medium used for cultivating CHO cellular extensively, and discloses the prescription of the culture medium.
本发明提供了一种用于大规模培养CHO细胞的无血清培养基。
The present invention provides a serum free medium used for cultivating CHO cellular extensively, and discloses the prescription of the culture medium.
本发明提供了一种用于大规模培养CHO细胞的无血清培养基。
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