基因工程菌生产的饲料酶，也叫重组酶(recombinant enzyme)，这项生物技术涉及可表达有特异多肽序列编码的特异互补DNA(cDNA)的克隆和分离，把cDNA转入到作为表达载体(expression vector)的...
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recombinant enzyme purification 重组酶纯化
purification of recombinant enzyme 重组酶的纯化
recombinant fibrinolytic enzyme 重组蛇毒纤溶因子
Volvariella volvacea;Subtractive cDNA library;Subtractive hybridization;Xylanase;β-Glucosidase;Chitin deacetylase;Cloning;Expression;Escherichia coli;Pichia pastoris;Purification;Recombinant enzyme;Characterization.
草菇;cDNA削减文库;差异杂交;木聚糖酶;β-葡萄糖苷酶;几丁质脱乙酰酶;克隆;表达;大肠杆菌;毕赤酵母;重组酶;纯化;酶学性质。
参考来源 - 草菇cDNA削减文库的构建及三种多聚糖降解酶基因的克隆与表达Volvariella volvacea;Subtractive cDNA library;Subtractive hybridization;Xylanase;β-Glucosidase;Chitin deacetylase;Cloning;Expression;Escherichia coli;Pichia pastoris;Purification;Recombinant enzyme;Characterization.
草菇;cDNA削减文库;差异杂交;木聚糖酶;β-葡萄糖苷酶;几丁质脱乙酰酶;克隆;表达;大肠杆菌;毕赤酵母;重组酶;纯化;酶学性质。
参考来源 - 草菇cDNA削减文库的构建及三种多聚糖降解酶基因的克隆与表达·2,447,543篇论文数据，部分数据来源于NoteExpress
The recombinant enzyme has several advantages, such as the better catalyzed ability and fewer producing expenses.
重组酶具有选择特异性更高的特点，与生化提取方法相比成本较低廉。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
The mutant enzyme showed its activity as 1.3 folds as the original recombinant enzyme with DL-hydantoin as the substrate, whilst as 2.4 times with Dp-hydroxyophenylhydantoin as the substrate.
进化酶催化底物海因水解的活性为亲本重组酶的1.3倍，催化底物对羟基苯海因水解的活性为亲本重组酶的2.4倍。
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