The recombinant enzyme has several advantages, such as the better catalyzed ability and fewer producing expenses.
重组酶具有选择特异性更高的特点，与生化提取方法相比成本较低廉。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
The mutant enzyme showed its activity as 1.3 folds as the original recombinant enzyme with DL-hydantoin as the substrate, whilst as 2.4 times with Dp-hydroxyophenylhydantoin as the substrate.
进化酶催化底物海因水解的活性为亲本重组酶的1.3倍，催化底物对羟基苯海因水解的活性为亲本重组酶的2.4倍。
To evaluate its heredity stability, the recombinant virus genome was extracted and digested by Hindlll every 5 generations, and 40 generations later, its enzyme digestion sites almost keep stable.
同时在连续传代40代后每隔5代提取重组病毒全基因组进行酶切鉴定，发现其酶切位点保持不变。
Conclusion The recombinant expression plasmid constructed by restriction enzyme cleave identification can highly express recombinant human ZP3 protein.
结论：经酶切鉴定构建的人透明带蛋白3重组表达载体可高效表达重组人zp3蛋白。
The reactivity of these recombinant proteins with sera from patients with amebiasis was examined by means of enzyme-linked immunoadsorbent assay (ELISA).
所获得的重组蛋白，用ELISA法检测它们与阿米巴病患者血清的反应性。
Results Enzyme digestion analysis and DNA sequencing showed that TROP2 targeted RNA interference recombinant plasmids were constructed successfully.
结果酶切鉴定和DNA测序分析显示，TROP2靶向RNA干扰重组质粒构建成功。
An indirect enzyme-linked immunosorbent assay (ELISA) was developed based on a purified recombinant F41 pili protein of enterotoxigenic Escherichia coli (ETEC).
以纯化的重组F41菌毛蛋白作为检测抗原，建立了检测产肠毒素大肠杆菌F41菌毛抗体的间接ELISA方法。
CONCLUSION Active recombinant human CYP2B6 obtained using baculovirus expression system will facilitate further examination of the role of this enzyme in drug metabolism.
结论杆状病毒表达系统能成功表达有活性的CYP2B6重组酶，可用来进一步研究在药物代谢中的作用。
AIM: to observe the inhibitory effect of luteolin on recombinant human protein kinase CK2 holoenzyme and to confirm the inhibitory type by its enzyme kinetic analysis in vitro.
目的：观察体外藤黄菌素对重组人蛋白激酶CK2的抑制效果及进行酶动力学分析以确定其抑制作用类型。
The positive recombinant plasmid was identified by PCR, enzyme digestion. The nucleotide sequence of CSP3 'ending gene was determined by the dideoxy chain termination method.
阳性重组质粒经pcr、酶切鉴定，用双脱氧链末端终止法进行序列测定。
The invention discloses a recombinant vector co-expressed by medicament metabolizing enzyme P450 2e1 of cynomolgus monkey and REDOX enzyme P450 of cynomolgus monkey.
本发明公开了一种食蟹猴P 4502e1药物代谢酶及其与食蟹猴p450氧化还原酶的共表达的重组载体。
Conclusion The recombinant GST-3C protease can be not only applied to the cleavage of fusion proteins, but also be used as a tool-enzyme in TAP system containing 3C recognition sequence.
结论GST-3C蛋白酶不仅可以应用于融合蛋白的酶切，而且为引入3C蛋白酶识别位点的串联亲和纯化（TAP）系统提供又一种酶切工具。
After culture optimization, the enzyme production of the recombinant strain was 3 times higher than that of the wild strain.
优化培养条件后，此重组菌的发酵酶活单位明显高于野生菌，最高达野生菌的3倍。
Results: the recombinant PS had nature activity. The system of enzyme activity was build and optimized. A novel PS inhibitor was found by the screening.
结果：得到了具有天然活性的泛酸合成酶，建立并优化了酶活测定体系及筛选模型，应用模型筛选得到了具有新结构的抑制剂。
Results: the recombinant PS had nature activity. The system of enzyme activity was build and optimized. A novel PS inhibitor was found by the screening.
结果：得到了具有天然活性的泛酸合成酶，建立并优化了酶活测定体系及筛选模型，应用模型筛选得到了具有新结构的抑制剂。
应用推荐