Objective to obtain the VH and VL variable region genes of murine McAbs to human CD molecule.
目的获取系列鼠抗人CD分子单抗的轻链及重链可变区基因并进行克隆和测序。
RESULTS: The VH and VL genes were homologous with the published gene sequences of mouse antibody variable region.
结果：克隆的基因序列符合小鼠轻、重链可变区基因的特征；
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT - PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
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