VH and VL genes 轻重链可变区基因
Objective to obtain the VH and VL variable region genes of murine McAbs to human CD molecule.
目的获取系列鼠抗人CD分子单抗的轻链及重链可变区基因并进行克隆和测序。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
RESULTS: The VH and VL genes were homologous with the published gene sequences of mouse antibody variable region.
结果：克隆的基因序列符合小鼠轻、重链可变区基因的特征；
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