Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.

通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。

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Both methods are in the research stage and require the cost of the DNA sequencing to drop even further before they could be considered for the clinic.

目前这两种检测方法尚处在研究阶段，在考虑进行临床运用之前，仍然有待于进一步降低DNA测序成本。

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Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.

方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。

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In the latest work, the team, from London and the University of Oxford, used DNA sequencing methods to study XMRV.

BBC: By Helen Briggs