Sandwich ELISA, dot blotting and DNA sequencing methods were used for the identification of positive clones.
通过硝酸纤维膜斑点印迹法进行阳性克隆鉴定。
Both methods are in the research stage and require the cost of the DNA sequencing to drop even further before they could be considered for the clinic.
目前这两种检测方法尚处在研究阶段，在考虑进行临床运用之前，仍然有待于进一步降低DNA测序成本。
Methods The mutation of mitochondrial DNA from all 18 family members of a chinese pedigree with maternally inherited aminoglycoside antibiotic-induced deafness was detected by PCR and DNA sequencing.
方法应用PCR和DNA序列分析技术，对一个有明确氨基糖苷类抗生素应用史的母系遗传耳聋家系共18人（包括聋人和听力正常者）的线粒体DNA进行研究。
Methods Genomic DNA was extracted from frozen tissues of 10 ameloblastomas and one malignant ameloblastoma. AMBN gene alterations were detected by PCR-direct sequencing.
方法收集10例成釉细胞瘤和1例成釉细胞癌的新鲜组织标本及相应外周血或牙龈黏膜组织，直接测序法分析AMBN基因；
Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.
方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。
The effects of DNA templates, primers, cycle sequencing reaction conditions, purification methods, operation of instrument were comparatively analyzed.
对测序中的模板、引物、测序反应条件及测序反应纯化方法和仪器操作等进行研究。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
Methods Molecular biology technologies of RT-PCR, directed cloning and DNA sequencing were carried out.
方法采用RT -PCR、定向克隆和DNA测序等一系列分子生物学技术进行本实验。
Methods PCR-STR and DNA sequencing technology were used to detect rare alleles at 15 STR loci for 4650 unrelated individuals.
方法应用PCR -STR和DNA序列分析技术，对4650个无关个体在15个STR基因座中的罕见等位基因进行检测。
Methods the effects of DNA purity and DNA concentration, as well as the quality of water used during sequencing on automated DNA sequencing results were retrospectively analyzed.
方法对DNA纯度、浓度和测序过程中所用水的质量对DNA自动测序结果的影响进行了回顾性研究。
Methods PCRSSCP and DNA sequencing were used to analyze the PTCH gene mutations in 12 OKCs, including 10 sporadic and 2 nevoid basal cell carcinoma syndrome (NBCCS) associated OKC.
方法采用PCR - SSCP筛查与DNA直接测序的方法对12例okc进行PTCH基因突变的检测，其中2例为痣样基底细胞癌综合征(NBCCS)相关okc,10例为散发okc。
The research methods in phylogeny of Bombus genus both at home and abroad was reviewed in the paper. The major research method included chromosome counts, protein analyzes, DNA sequencing.
本文就目前国内外熊蜂属系统进化领域的分子生物学研究方法进行了综述，主要包括染色体计数法、蛋白质分析法、DNA测序法。
Methods immunohistochemistry in 52 cases of nasopharyngeal carcinoma PTEN gene expression, the use of PCR SSCP silver staining, DNA sequencing analysis to detect PTEN gene mutation in exon 5, 8.
方法采用免疫组化检测52例鼻咽癌中pten基因的表达，利用PCRSSCP银染、DNA测序分析等方法检测pten基因第5、8外显子突变。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（ RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
New methods of sequencing degraded fragments of DNA allowed scientists to make the first direct comparisons between the modern human genome and that of our Neandertal ancestors.
对DNA降解片段进行测序的新方法使得科学家们能够第一次对现代人基因组与我们的尼安德特人祖先的基因组进行直接的比较。
Methods Bi-directional DNA sequencing was used to detect the ECM1 gene in patients with LP as well as among their parents, siblings and children.
方法采用DNA双向测序法直接检测类脂质蛋白沉积症一家系中患者及其父母、同胞、子女的ECM1基因。
Methods The effects of DNA templates, primers, cycle sequencing reactions as well as purification methods were comparatively analyzed.
方法对测序中模板、引物、测序反应条件及其产物的纯化等因素进行比较研究。
Methods Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing.
方法从确诊的HIV - 1感染者全血样本中提取基因组dna，经套式聚合酶链反应(PCR)扩增和测序。
Methods TLR2 gene polymorphisms were analyzed by DNA sequencing in a small amount of samples. SNPs in TLR2 gene were detected by ligase detection technique in a large amount of samples.
方法在小样本中测序检测TLR2基因中可能存在的基因多态性，再用连接酶特异检测技术在大样本进行SNP分型，并通过统计学方法分析基因多态性与结核病易感性之间相关性。
Sequencing-by-synthesis methods simulate parts of this process on a single DNA strand of interest.
合成定序法模拟细胞的复制步骤，分析单股DNA的序列。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods A patient was diagnosed as Darier s disease by pathology and clinical features. PCR and DNA sequencing were used to detect the gene mutation of the family.
方法1例经组织病理结合临床诊断为毛囊角化病，采用聚合酶链反应和DNA测序方法对此家系进行基因突变情况检测。
Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
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