方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
方法：收集健康新鲜的前磨牙，按年龄分组，石蜡包埋切片，免疫组织化学与图像定量分析。
Methods: Fresh health premolars were collected, divided into some groups according to age, embedded in paraffin and cut into slices.
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