方法免疫组织化学染色结合显微图像定量分析和免疫荧光双重染色。
Methods Immunohistochemical staining combined with the micro-image analysis and immunofluorescence histochemical double-staining technique were used.
方法应用免疫组织化学方法检测erk在脊髓后角的定位表达，并用图像分析系统和免疫印迹法进行定量分析。
Methods The expression of ERK in spinal dorsal horn was detected by immunohistochemical method and was quantitated by western-blot analyses.
方法：收集健康新鲜的前磨牙，按年龄分组，石蜡包埋切片，免疫组织化学与图像定量分析。
Methods: Fresh health premolars were collected, divided into some groups according to age, embedded in paraffin and cut into slices.
免疫组织化学染色片显微镜下放大200倍，每个标本取6张切片，随机取5个视野，计算免疫组织化学染色的平均吸光度值和积分吸光度值进行半定量分析。
Under the microscope magnifying 200,6 sections of each sample in 5 random sights were selected to conduct the semi-quantitative analysis of the mean absorbance (Am) and integral absorbance (Ai).
方法石蜡包埋切片，应用免疫组织化学SP法及麦克奥迪真彩色病理分析图像分析系统，对TTF-1表达强度的阳性单位（PU）进行定量分析。
Methods The TTF-1 expression intensity (in positive units, PU) in paraffin-embedded tissue blocks was determined using immunohistochemical SP method in combination with Motic Images System.
方法石蜡包埋切片，应用免疫组织化学SP法及麦克奥迪真彩色病理分析图像分析系统，对TTF-1表达强度的阳性单位（PU）进行定量分析。
Methods The TTF-1 expression intensity (in positive units, PU) in paraffin-embedded tissue blocks was determined using immunohistochemical SP method in combination with Motic Images System.
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