常用的血清学检测方法为酶联免疫吸附法(ELISA)或在此基础上改进的斑点酶联免疫吸附法(Dot-Blot ELISA)等。Dot-Blot ELISA具有快速和能检测大量样品的特点,在大量检测样品时较常应用。
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Tissue blot-ELISA 组织印迹法
Dot blot-ELISA 酶联免疫吸附试验
dot-blot ELISA 印迹酶联免疫吸附法
Indirect-blot ELISA 间接斑点ELISA
Western blot ELISA 蛋白质免疫印迹酶联免疫吸附法
indirect blot elisa 间接斑点elisa
indirect - blot elisa 间接斑点elisa
Western Blot ELISA immunofluorescence 应用范围
ELISA Western Blot 应用范围
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
间接免疫荧光、ELISA和免疫印迹检测证明,含SO . 7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
蛋白印迹实验鉴定转基因植株,ELISA方法测定重组蛋白表达水平,集落形成实验测定重组蛋白活性。
The results showed recombinant NP protein could bind to both positive serum and MAb with specificity in ELISA, but only bind to positive serum in western blot.
结果表明:所获得的重组NP蛋白在ELISA分析中均能与阳性血清和单克隆抗体发生特异性结合,但在免疫印迹分析中仅与阳性血清发生特异性结合。
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