By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
间接免疫荧光、ELISA和免疫印迹检测证明,含SO . 7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
PCR and Western blot were used to select the transgenic tobacco plants. ELISA was used to evaluate the expression level while colony forming assay was used to test its biological activity.
蛋白印迹实验鉴定转基因植株,ELISA方法测定重组蛋白表达水平,集落形成实验测定重组蛋白活性。
Its immunoreactivity was analyzed by Western blot, ELISA and blocking ELISA using MAb and positive serum.
采用单克隆抗体和阳性血清经elisa、阻断elisa、免疫印迹分析重组蛋白的免疫反应性。
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