【Key words】 Pyrus pyrifolia Nakai； Adventitious bud regeneration； ACC Oxidase； Regulatory sequence； Clone； Lipoxygenase； ACO interference gene； LOX interference gene； Agrobacterium mediated； Tomato； Tobacco；
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Agrobacterium-mediated transformation 农杆菌介导法 ; 农杆菌转化 ; 农杆菌介导转化法 ; 转化方法
Agrobacterium-mediated method 农杆菌介导法
Agrobacterium-mediated DNA transfer 农杆菌介导的DNA转化
sonication-assisted agrobacterium-mediated transformation 农杆菌介导法 ; 声波辅助农杆菌遗传转化
Agrobacterium-mediated 农杆菌介导法
Agrobacterium-mediated system 根农杆菌介导法
At present, Agrobacterium mediated transformation of leaves are widely adopted in apples, and transgenic shoots were mainly obtained from adventitious bud regeneration.
目前，农杆菌介导法是苹果上应用的主要转化方法，叶片再生不定芽途径是被广泛应用的受体系统。
Sonication assisted Agrobacterium mediated transformation(SAAT)tremendously improves the efficiency of Agrobacterium infection by introducing large numbers of microwounds into the target plant tissue.
由于超声波处理可增加农杆菌与外植体之间的接触面积，提高农杆菌对外植体的转化频率。
The review introduced the theory and methods of Agrobacterium-mediated transformation of peanut, and the factors influencing transformation efficiency were discussed.
介绍农杆菌介导的花生遗传转化的原理和方法并对影响转化率的因素进行了探讨。
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