At present, Agrobacterium mediated transformation of leaves are widely adopted in apples, and transgenic shoots were mainly obtained from adventitious bud regeneration.
目前,农杆菌介导法是苹果上应用的主要转化方法,叶片再生不定芽途径是被广泛应用的受体系统。
A stable and efficient Agrobacterium mediated transformation system has been established which offers a base for high yield, high quality and broad resistance of disease Sugarbeet variety.
本项研究初步建立了一种转化频率高、稳定性好、通用性较强的甜菜遗传转化技术体系,从而通过植物转基因技术为选育高产、优质、广谱抗病的甜菜品种奠定基础。
Sonication assisted Agrobacterium mediated transformation(SAAT)tremendously improves the efficiency of Agrobacterium infection by introducing large numbers of microwounds into the target plant tissue.
由于超声波处理可增加农杆菌与外植体之间的接触面积,提高农杆菌对外植体的转化频率。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥,筛选抗性转基因拟南芥植株,进行PCR检测和GUS染色。
The review introduced the theory and methods of Agrobacterium-mediated transformation of peanut, and the factors influencing transformation efficiency were discussed.
介绍农杆菌介导的花生遗传转化的原理和方法并对影响转化率的因素进行了探讨。
Applying to leaflet regeneration system of peanut, genetic transformation of peanut using Agrobacterium-mediated approach were carrying on the research.
应用花生胚小叶再生体系,以农杆菌介导法对质粒上的基因转化进行初步探讨。
In this study, a high efficient Agrobacterium-mediated transformation system has been established and a large-scale rice enhancer trapping population has been generated.
建立水稻突变体库是功能基因组学研究的有效手段,而高效的水稻转化体系是建立突变体库的重要基础。
The soybean ferritin gene can be transferred into upland rice using the method of Biolistic and Agrobacterium-mediated transformation, which increased iron concentration in the transgenic plant seeds.
利用基因枪和农杆菌转化法可将大豆铁结合蛋白基因导入旱稻基因组中,并使转基因稻米铁含量增高。
The factors influencing the frequency of rice transformation mediated by Agrobacterium have been investigated by using 16 commercially important indica and japonica rice cultivars or lines.
以16种重要的籼稻和粳稻栽培品种为材料,研究了影响农杆菌转化水稻频率的有关因素。
The common method of Genetic Transformation of Brassica campestris L. is Agrobacterium-mediated method.
白菜遗传转化技术中最常用的方法是农杆菌介导法。
Therefore, carbenicillin was the preferable choice of the two tested antibiotics for Agrobacterium tumefaciens-mediated transformation of tall fescue.
因此,在农杆菌介导高羊茅遗传转化中,抑菌剂以选用羧苄青霉素较为合适。
The present invention improves the callus of inducing and the bud inducing of the explants, and enhances the agrobacterium-mediated ability.
本发明方法改良了外植体愈伤和生芽的诱导,提高了农杆菌介导的能力。
An efficient and stable Agrobacterium-mediated genetic transformation system for Torenia was established through investigating the factors influencing the transformation efficiency.
通过对影响根癌农杆菌介导蓝猪耳转化效果各种因素的研究,建立了蓝猪耳高效稳定的遗传转化系统。
The Agrobacterium-mediated transient expression analysis in intact tissues has emerged as a rapid and useful method to study gene silencing in plants.
农杆菌介导的瞬时表达体系是一个研究整株植物基因沉默的快速实用系统。
The tomato iron transporter gene (LeIRT2) was introduced to Malus robusta Rehd. via Agrobacterium-mediated transformation to produce iron-deficiency tolerant apple rootstock.
用农杆菌介导法,成功地将番茄铁转运蛋白基因导入了苹果砧木八棱海棠。
The tomato iron transporter gene (LeIRT2) was introduced to Malus robusta Rehd. via Agrobacterium-mediated transformation to produce iron-deficiency tolerant apple rootstock.
用农杆菌介导法,成功地将番茄铁转运蛋白基因导入了苹果砧木八棱海棠。
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