无血清体外培养树突状细胞的研究 关键词】 树突状细胞；无血清培养液；钙离子载体；外周血 [gap=1784]Key wordsdendritic cell; serum-free medium; calcium ionophore; peripheral blood
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将RPE细胞以1times;106个细胞接种于10cm的培养皿中，培养72h后更换为无血清培养基(serum-free medium, SFM)培养。实验分阻断剂处理组和对照组。
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serum free medium 无血清培养基 ; 血清培养基 ; 无血清培养液 ; 无血清培养
serum free medium SFM 无血清培养基
keratinocyte serum free medium 细胞培养基 ; 无血清角质细胞培养液 ; 培养基
General Purpose Serum-free Medium 通用无血清培养基
defined keratinocyte-serum free medium 无血清培养基 ; 以无血清培养基
LONZA 12-725F UltraCULTURE Serum-free Medium UltraCULTURE无血清培养基产品
keratinocyte serum-free medium 细胞无血清培养基 ; 培养基
Serum - free medium 无血清培养液
serum-free medium sfm 无血清培养基
Objectives: To study whether the human breast cancer line MCF-7 contains breast tumor stem cell and its growing patten in serum-free medium supplemented with mitogens.
目的研究无血清培养基悬浮培养MCF-7乳腺癌细胞系，筛选并鉴定MCF-7乳腺癌细胞系中的肿瘤干细胞相关亚群。
参考来源 - 无血清悬浮法培养MCF·2,447,543篇论文数据，部分数据来源于NoteExpress
The model of dissociated cell cultures of human fetal pituitary in serum-free medium was established.
采用人胎脑垂体前叶分散细胞建立了无血清体外培养模型。
It was demonstrated that pituitary cells could survival and synthesize and release hormones in serum-free medium.
结果表明分散垂体细胞在无血清培养液中能够存活，并具有合成和分泌激素的功能。
Results: The spiral ganglion neurons could survive and had a normal phenotypic differentiation in serum-free medium.
结果：显示耳蜗螺旋神经节神经元在体外无血清培养条件下，可以存活并进行正常分化。
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