The model of dissociated cell cultures of human fetal pituitary in serum-free medium was established.
采用人胎脑垂体前叶分散细胞建立了无血清体外培养模型。
It was demonstrated that pituitary cells could survival and synthesize and release hormones in serum-free medium.
结果表明分散垂体细胞在无血清培养液中能够存活，并具有合成和分泌激素的功能。
Results: The spiral ganglion neurons could survive and had a normal phenotypic differentiation in serum-free medium.
结果：显示耳蜗螺旋神经节神经元在体外无血清培养条件下，可以存活并进行正常分化。
Western blot analysis of partially purified, concentrated, serum-free medium containing human MMP-9, using MMP-9 Antibody.
含有人MMP - 9的无血清培养基经过部分纯化，浓缩后使用MMP - 9抗体进行western blot分析。
We report a novel three-stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum-free medium.
我们提出了一种新颖的三阶段方法有效地指示的人胚胎干细胞的分化成肝细胞在无血清培养基中。
Methods: We selected the best medium fitted for es cells culture, and analyzed the neural differentiation of es cells by comparing increased number of viable cells among different serum-free medium.
方法：比较ES细胞在不同培养基中的生长情况，分析ES细胞在不同时间分化形成神经细胞的比例。
Presumptive zygotes were cultured in mSOFaa medium supplemented with OCS or FBS had higher blastocyte yield than that in culture medium with free serum.
受精卵在含血清FBS或OCS培养液中的囊胚发育率明显高于在无血清培养基中的囊胚发育率。
During the later period of the cultivating process, the applying of serum free medium had no significant effects on the cell growth and protein expressing.
在培养后期换用无血清培养基培养，细胞生长及蛋白表达水平正常。
Instect cell culture media were optimized carefully to find new medium for special cell strain and serum free media was manufactured commercially for suspension cell mass production;
通用的昆虫细胞培养基配方的不断优化，特定昆虫细胞株培养基的研制和适用于大规模悬浮培养的无血清培养基的商品化；
Methods Human keratinocytes were primarily cultured in vitro with serum free medium.
方法以无血清培养基体外原代培养角质形成细胞。
Conclusion At present, the serum-containing medium for culture of CHO cells can not be completely replaced by serum-free serum.
结论目前无血清培养基尚不能完全取代含血清培养基用于培养CHO细胞。
The present invention provides a serum free medium used for cultivating CHO cellular extensively, and discloses the prescription of the culture medium.
本发明提供了一种用于大规模培养CHO细胞的无血清培养基。
METHODS: the influence of he ne laser irrigation on the growth and development of neurons in hippocampus and cortex of frontal lobe neurons was observed int he cell culture of serum free medium.
方法：采用无血清培养的新生大鼠海马神经元和额前叶皮质神经元，观察氦氖激光照射对神经元生长发育的影响。
Objective to explore the optimal condition of direct differentiation into dopaminergic neurons of embryonic stem cells in serum-free and feeder layer cell free medium.
目的寻求无血清、无饲养层细胞存在的情况下，胚胎干细胞向多巴胺能神经元定向诱导分化的最佳条件。
This paper suggested that KSOM can be used as serum free medium for ovine embryo development in vitro.
说明KSOM可以用于绵羊胚胎早期发育的无血清培养液。
AIM to observe the effects of epidermal growth factor (EGF) and bovine pituitary extract (BPE) on human pituitary adenoma cells cultured in serum free medium.
目的观察表皮生长因子(EGF)和牛垂体提取物(BPE)在人垂体腺瘤细胞培养中的作用。
Methods kidney cortex explanted into the serum-free culture medium gave rise to a primary culture of kidney epithelial cells. After confluence, cells were subcultured using Trypsin-EDTA digestion.
方法取肾皮质组织块，应用无血清培养液进行原代培养，融合后用胰蛋白酶-EDTA消化传代。
The rainbow trout embryo cell line medium cultured by the serum-free rainbow trout embryo cell line medium of the invention has the advantages of rapid breeding speed and long regenerating cycle.
本发明无血清虹鳟胚胎细胞系培 养基培养虹鳟胚胎细胞系具有增殖速度快，传代时间长的优点。
Conclusions With the application of the "m" serum - free medium, the technique of Schwann cell culture was simplified and the contamination of fibroblasts was effectively controlled.
结论应用“M”无血清培养液简化了雪旺细胞的培养技术，有效地控制了成纤维细胞的污染。
Conclusion: 4t1 cells can grow and form spheres in serum-free suspension medium containing growth factor, and they contain breast cancer stem-like cells, which can be enriched when cultured in SFM.
结论：乳腺癌4t 1细胞可在含多种生长因子的SFM中悬浮生长并形成细胞球，4t1细胞中含有的乳腺癌干细胞样细胞可通过SFM培养法富集。
Serum-free DMEM medium with high glucose works for us.
我们使用的是不含血清的高糖DMEM培养基，效果很好。
Serum-free DMEM medium with high glucose works for us.
我们使用的是不含血清的高糖DMEM培养基，效果很好。
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