目的构建HP450基因的克隆载体。
本文描述一种构建与P CR产物直接连接的克隆载体的方法。
A new method for construction of a cloning vector for direct ligation with PCR products was described.
目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
目的建立风疹病毒包膜糖蛋白e1的克隆载体；研究e1基因变异情况，并对其序列进行系统发生树分析。
Objectives to construct the cloning vector of glycoprotein E1 gene of rubella virus, to study the mutation of glycoprotein E1 gene, and to analyze the sequence of E1 gene by the phylogenetic tree.
细菌人工染色体(BAC)是一种承载dna大片段的克隆载体系统，用于人、动物和植物基因组文库构建。
Bacterial artificial chromosome (BAC) is a kind of vector system used to construct large fragment insert libraries of genome DNA in human, animals and plants.
人血管生成素-1基因的克隆及表达载体的构建，为进一步研究其功能、活性和应用奠定了基础。
The cloning of human angiopoietin 1 gene and construction of it's expression vectors lay a good foundation of further study on the function, biological activity and application.
结果：将编码人单核细胞趋化蛋白—1（MCP—1）基因克隆至融合表达载体pGEX—2T中，DNA测序证实正确。
Results: A gene fragement encoding MCP-1 was cloned into the fusional expression vector PGEX-2T. DNA sequencing indicated that it was correct.
在腺病毒载体的构建过程中，早期和晚期启动子的选择、构建以及去除末端蛋白(TP)、克隆末端序列是至关重要的。
The selection and cloning of early and late promoters and removal of adenovirus terminal protein (TP) are very important in construction of adenovirus vector.
目的：构建血管基膜衍生多功能肽克隆和原核表达载体，并对血管基膜衍生多功能肽氨基酸序列进行空间结构分析预测。
Objective: to construct cloning and prokaryotic expression vector of vascular basement membrane-derived multifunctional peptide and to analyze its space conformation.
目的克隆大鼠水通道蛋白4（AQP4）M23基因并构建其载体为进一步研究提供理论基础和研究工具。
Purpose: AQP4 M23 gene cloning and construction in the expression vector can provide us a research tool.
结果酶切和测序证实PTEN基因克隆和真核表达载体构建成功。
Results Restriction enzyme analysis and DNA sequence analysis showed that PTEN gene was cloned and the eukaryotic expression vector was constructed successfully.
本研究的主要目的是克隆芽孢杆菌胞外高丰度蛋白的表达元件，为今后构建表达载体奠定基础。
The objective of this research is to clone the expression element of a major secretory protein gene from Bacillus sp.
分别转染膀胱癌细胞株EJ ，观察不同载体对膀胱癌细胞体外增殖、克隆形成和细胞周期时相的影响。
The influence of each vector on the cell proliferation, clone-formation and alteration of cell cycle of bladder cancer cell line EJ were observed in vitro.
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
目的克隆激活转录因子(atf)5，构建其真核表达载体，观察其在细胞中的表达定位。
Objective to clone the gene activating transcription factor 5 (ATF5), construct the expression plasmid and detect the localization of ATF5 in cultivated cells.
有时孩子看起来完全是一个克隆的父母载体。
Sometimes it looks like the kid is totally a clone of one of the parents.
结论t -载体法是克隆pcr产物通用而有效的方法。
Conclusion it is a general and effective method to clone PCR products with T-vectors.
序列分析表明所克隆的SCF序列与文献报道的一致，构建的表达载体经鉴定正确。
The sequence of the cloned SCF is same as the published report. The construction of expression plasmid is identified in a correct way.
为了产生这种重组分子，载体和要克隆的DNA必须在特异位点切开，并以可控制的方式连接起来。
To produce this recombinant molecule, the vector, as well as the DNA to be cloned, must be cut at specific points and then joined together in a controlled manner.
结果从活化的人白细胞中克隆到IDO基因编码区全长，并构建了IDOEGFP融合蛋白表达载体。
Results The full-length coding sequence of IDO was cloned from activated human leukocytes and the expression vector for IDO-EGFP fusion protein was constructed.
目的克隆人TRAIL基因，构建其原核表达载体。
To clone gene of the TRAIL and construct its prokaryotic expression vector.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
结果成功引入点突变并构建置换型打靶载体，转染es细胞后经药物筛选得到抗性细胞克隆。
The drug resistant cell clones were picked after drug selection. Results The construction of targeting vectors was successful and several drug resistant es cell clones were gained.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
目的克隆人热休克蛋白70 (HSP70)基因，构建其原核高效表达载体。
Objective to clone human heat shock protein 70 (HSP70) gene for the construction of a prokaryotic expression vector.
目的克隆人血管紧张素转换酶2基因（ACE2），并构建其真核表达载体。
Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid.
目的：构建高效表达人透明带蛋白3的真核重组表达载体。方法：利用PCR、T- A载体克隆和亚克隆等技术。
Objective To construct the recombinant plasmid that highly expressed human zona pellucida 3. Methods The techniques of PCR amplification, T-A vector ligation, and sub-clone were used.
方法将特异性引物从S8546病毒感染细胞PCR扩增的产物克隆于T载体，正确的克隆纯化后测序，应用DNASTAR软件比较分析。
Methods RT PCR products were amplified from S85 46 virus infected cells, cloned into T vector, sequenced and analyzed using DNASTAR software.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
本研究旨在克隆通城猪含有第1个内含子的MSTN前肽基因，构建真核定点诱变载体，并通过转染C2C12细胞验证载体表达的有效性。
This research intended to construct eukaryotic expression vector with a site-directed mutation of porcine MSTN propeptide gene, and verify its expression efficacy in C2C12 cells.
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