克隆载体（Cloning Vector）：通常采用从病毒、质粒或高等生物细胞中获取的DNA作为克隆载体，在载体上插入合适大小的外源DNA片段，并注意不能破坏载体的自我复制性质。将重组后的载体引入到宿主细胞中，并在宿主细胞中大量繁殖。常见的载体有质粒，噬菌粒，酵母人工染色体。
克隆载体 [kè lóng zǎi tǐ]
网络释义专业释义
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cloning vector - 引用次数：9
The target fragment and linear plasmids were obtained from the cloning vector T-CBF2 and from the plant expression vector PBI121 with dual digestion using BamHⅠand Sac I,respectively.
用BamHⅠ和SacⅠ分别对克隆载体T-CBF2和植物表达载体PBI121进行双酶切,获得目的片段和线性质粒。
参考来源 - 抗冻基因CBF -
cloning vehiclecloning vectorclonal vehicle
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双语例句原声例句
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目的构建HP450基因的克隆载体。
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本文描述一种构建与P CR产物直接连接的克隆载体的方法。
A new method for construction of a cloning vector for direct ligation with PCR products was described.
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目的构建克隆载体，分析隐匿性乙型肝炎病毒S基因的突变情况，构建其原核融合蛋白表达载体。
Objective To construct the clone vector of S gene in occult hepatitis B virus infection. To analyse its mutations and to construct its prokaryotic expression vector.
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Let's assume that we have this plasmid cloning vector and we have some pieces of DNA that we would like to put into a plasmid that we would like to make copies of.
假设我们已经有了克隆质粒载体,也有了我们想要将之,整合到质粒上的某些DNA片段,同时也是我们所要复制的DNA片段
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