①目的构建重组蜂毒肽的原核表达克隆。

Objective To construct mellitin gene expression clone.

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结论：可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。

Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.

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用新霉素类似物(G418)筛选出阳性克隆，并用流式细胞术进一步筛选出高效表达克隆，建立稳定高效表达CHO细胞株。

The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.

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用新霉素类似物(G418)筛选出阳性克隆，并用流式细胞术进一步筛选出高效表达克隆，建立稳定高效表达CHO细胞株。

The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.

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