Took it out of the virus completely, cloned it into a plasmid, that plasmid was expressed in a foreign host, in this case it was expressed in yeast cells.

将基因从病毒中完全提取出来,并克隆制成质粒,该质粒在外来宿主中表达,此例中质粒在酵母细胞中表达

耶鲁公开课 - 生物医学工程探索课程节选

We're going to have DNA that we would like to make lots of copies of, or we're going to have DNA that we would like to get expressed in a cell, and we're going to put it in this region that's called the polylinker.

我们将那些需要得到很多拷贝数的DNA,或者需要在细胞中表达的DNA,整合到这个叫做多克隆位点的区域中

耶鲁公开课 - 生物医学工程探索课程节选