目的：研究不同药物浓度、不同化学结构的氟喹诺酮类药物对突变选择窗(MSW)内筛选的大肠埃希菌耐药突变体的靶位耐药基因的影响。
OBJECTIVE To investigate the effect of drug concentration, drug structure of fluoroquinolones on the resistant gene of Escherichia coli mutants selected in the mutant selection window (MSW).
此外，尚有10个氨基酸替代散在于分子内其它区域，这种替代是由于基因突变造成的。
Scattering on other regions of the molecule there were 10 single amino acid substitutions, which could be explained as site-mutations of the gene.
很多人类疾病在家族内遗传，起因于单个基因突变。
Many human diseases are inherited in families and result from mutations in single genes.
目的用克隆法研究放射性核素内照射诱发的大鼠外周血淋巴细胞HPRT基因突变情况。
Objective to study HPRT gene mutation in rat peripheral blood lymphocytes induced by internal exposure to radionuclides using cloning method.
EIN3基因首先是从拟南芥的突变体中克隆得到的，作用于CTR下游，位于核内，编码转录因子。
EIN3 was first cloned in Arabidopsis thalina mutant, and it has been suggested that EIN3 acted downstream to CTR located in nuclear and encoded transcription factors.
移码突变和异义突变都能被基因内抑制基因所抑制。
Both frameshift and missense mutations can be suppressed by intragenic suppressors.
目的研究乙型肝炎（乙肝）病毒核心基因内缺失突变株（ C I D）在慢性乙肝及肝癌患者中存在的状态及意义。
Objective To study the status and significance of hepatitis B virus core internal deletion (CID) mutants in chronic hepatitis B and hepatocellular carcinoma patients.
这一技术不需要内切酶消化和连接酶处理，技术操作简单易行，在基因拼接、基因内部突变方面具有良好的应用价值。
The technique didnt need restrict enzymes and ligases, which could be developed as a simple and useful technique in the studies of gene splicing and gene mutation.
在APC基因突变的肿瘤细胞内，CTPB不被破坏，而是积聚在细胞内。
In tumor cells with mutated APC, CTPB is not destroyed; instead it accumulates in the cell.
在一定剂量范围内，人外周血淋巴细胞克隆效率与照射剂量呈负相关，HPRT基因突变频率与照射剂量呈正相关。
In the radiation dose range used, the colony efficiency of the lymphocytes was negatively correlated to the radiation dose, while the HPRT mutation frequency is positively correlated.
当研究人员向突变细胞内加入人造泛素——一种组蛋白复合物后，细胞恢复正常，表明这的确是Brca1基因的核心功能。
When the researchers added artificial ubiquitin-histone complexes to the mutant cells, the cells recovered, suggesting this was indeed the Brca1 gene's core function.
这种技术可在DNA靶标分子的任意位点进行基因敲除、敲入、点突变等操作，无需使用限制性内切酶和连接酶。
The Red mediated recombination can be used to insert, delete or substitute DNA sequences at any desired position on a target molecule without the need for restriction enzymes or DNA ligases.
方法：采用PCRSSCP法检测血管网织细胞瘤中VHL基因的突变率及甲基化，敏感限制性内切酶消化法检测血管网织细胞瘤中VHL基因的异常甲基化率。
Methods: The hypermethylation was examined by methyl sensitive restrictive DNA endoenzyme analysis in 34 cases of angioreticuloma and the VHL gene mutations detected by PCR SSCP analysis.
两个突变发生在同一基因内的抑制，叫基因内抑制。
Both frameshift and missense mutations can be suppressed by intragenic suppressors.
两个突变发生在同一基因内的抑制，叫基因内抑制。
Both frameshift and missense mutations can be suppressed by intragenic suppressors.
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