①目的构建重组蜂毒肽的原核表达克隆。
结论:可通过四环素抗性筛选系统筛选外源基因的高效表达克隆。
Conclussion: We could screen high expressed clone of heterologous gene using tetracycline resistance screening system.
用新霉素类似物(G418)筛选出阳性克隆,并用流式细胞术进一步筛选出高效表达克隆,建立稳定高效表达CHO细胞株。
The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.
用新霉素类似物(G418)筛选出阳性克隆,并用流式细胞术进一步筛选出高效表达克隆,建立稳定高效表达CHO细胞株。
The positive clones were selected by G418, and highly expressing clones were more selected by flow cytometry, and get stable highly expressing CHO cell lines.
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