对考染后切取了高丰度蛋白质点后的胶块再进行快速银染,可以既提高鉴定的准确性也不丢失低丰度蛋白质。
Fast silver staining following coomassie blue staining can not only improve the accuracy of protein identification, but also identify more low-abundance proteins.
本研究的主要目的是克隆芽孢杆菌胞外高丰度蛋白的表达元件,为今后构建表达载体奠定基础。
The objective of this research is to clone the expression element of a major secretory protein gene from Bacillus sp.
统计结果显示,贮藏蛋白以及相应的核糖体蛋白,起始因子,延伸因子以及转录因子等基因高丰度表达。
Of them, most highly-expressed genes encoded storage proteins, starch, ribosome proteins, initiation factors, elongation factors and transcription factors.
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