目的探讨细胞形态学检查抽取骨髓液量多少与骨髓标本质量之间的关系。
Objective Discussion cytomorphology inspection extraction marrow liquid volume how many and between marrow specimen quality relation.
方法:抽取兔髂骨及胫骨的骨髓液,采用密度梯度离心法结合贴壁培养法分离纯化出MSC,并增殖。
Methods: MSC were aspirated from rabbit iliac and tibias crest and purified by gradient centrifuge and adhesive culture methods.
方法:抽取兔髂骨及胫骨的骨髓液,经密度梯度离心法及塑料板贴壁培养法分离纯化出msc,并增殖。
Methods: MSC were harvested from iliac and tibias crest and obtained by using gradient centrifuge and adhesive culture methods.
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