饲养细胞(Feeder cell),也叫滋养细胞,在体外的细胞培养中,单个的或数量很少的细胞不易生存与繁殖,必须加入其它活的细胞才能使其生长繁殖,加入的细胞即为饲养细胞。
因此,科学家们在这项研究中,采用超过40天的药物处理,用人类胚胎干细胞种植饲养细胞。
So the scientists in this study, employing a pharmacological treatment over 40 days, seeded feeder cells with human embryonic stem cells.
目的: 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞,优化培养条件,建立饲养细胞系,为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞,将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
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